Ensaf Hassan Akel(1)* , Hanan kawas(1), Qusay Al-Rhayeh(1), Rehab Hamdan(1)and Imad D. Ismail (2)
(1). General Commission Agriculture Research, Lattakia, Syria
(2).Tishreen university, Faculty of Agriculture, Department of plan protection Lattakia, Syria
(* Corresponding author: Email: firstname.lastname@example.org )
Received: 07/06/2020 Accepted: 02/07/2020
This study was conducted to evaluate the efficiency of tow strains of Plant Growth Promoting Rhizobacteria, Pseudomonas chlororaphis MA342, and Bacillus subtillis FZB27, on reducing infection by Tomato yellow leaf curl virus (TYLCV), in tomato plants grown under protected agriculture, in Agricultural Research Centre during Autumn and Winter of 2018-2019 growing seasons. Tomato seeds were immersed in suspension of tow strains (concentration 9× 109 Bacterial cell/ml) before seeding, Also Tomato seedling before transplanting were treated with bacterial suspension (5ml/seedling). Additional 10ml/plant of bacterial suspension were added after 10 days according to the experiment treatments. The following were estimated: peroxides enzyme activity, viral concentration, percentage of viral inhibition. Results showed increasing in peroxides enzyme activity after 10 days in plants inoculated with TYLCV and treated with bacterial suspension B27 (0.032 µm /mg) and this was better than other used treatments MA and virus( 0.031 µm /mg) and control treatment (0.023 µm/mg). After 30 days of viral infection the peroxides enzyme activity was higher in plants treated with MA (1.362 µm /mg) than plants treated with B27 (0.940 µm/mg) and control treatment (0.449 µm/mg). Results of estimation of viral concentration by TAS-ELISA has shown decreasing in TYLCV concentration, after 50 days of inoculation. The absorbance values at 450 nm of extracts from MA treated plants (0.373) and B27 treatment plants (0.378) comparing with absorbance values (0.702) of extracts from control treatment. The percentage of viral inhibition in treated plants with MA (46.86%) was higher from plants treaded with B27 (46%) may be because the activity of peroxides enzyme was higher in plants treated with MA than others.
Key words: Pgpr, Tylcv, Tas- Elisa, peroxides Enzyme.
Full paper in Arabic: PDF