Detection the Ability of Some Local Fungal Isolatesin the Secretion of Hydrolysis Enzymes and Indol-3-Acetic Acid Growth Regulator

Zahra Ibrahim El-Gali*(1), and Amina Abdul-Hamied Saad(1)

(1).Department of Plant Protection, Faculty of Agriculture, Omer Al-Mukhtar University, El-Beida, Libya.

(*corresponding, Zahra El-Gali, E. mail:

Received: 19/09/2020                              Accepted:15 /10/2020


This study was conducted during the year 2018-2019 in plant pathology laboratory, Faculty of Agriculture, Omer Al-Mukhtar University, with the aim of detection the ability of some local isolates fungi Botrytis cinerea, Macrophominaphaseolina, Rhizoctoniasolani, Sclerotiniasclerotiorumand Trichodermaharzianum to produced hydrolysis enzymes such as cellulose, amylase and pectinase in solids media, as well as testingtheir ability to Indol-3-acetic acid (IAA) growth regulator secretion in Czapek brothmedia amended with tryptophan. Observations showed that fungal species were significantly differed for their ability to produce lytic enzymes. All the isolated fungi had the capacity to cellulase enzyme production in solid media and the all of them also were able to amylase enzyme production, except B. cinerea, while T. harzianumwas be not produce of pectinase enzyme. harzianum gave the highest capability for the production of cellulase (2.9cm) and amylase(4.5cm) enzymes, while M. phaseolinagave the highest (2.4cm) in pectinase production. The production ability was reflected on degradation indicator and degree of degradation. Concerning with growth regulator the results showed that T. harzianum only was able on Indol-3-acetic acid secretion.

Keywords: Fungi, lytic enzymes, growth regulators, IAA, Libya.

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