Amina Abdul-Hamied Saad(1) and Zahra Ibrahim El-Gali*(1)
(1). Department of Plant Protection, Faculty of Agriculture, Omer Al-Mukhtar University, Libya.
(*Corresponding author: Dr. Zahra Ibrahim El-Gali. E-Mail: Zahra.Ibrahim@omu.edu.ly).
Received: 22/06/2020 Accepted: 18/07/2020
Abstract
The study was conducted In Vitro during 2018-2019 at Plant Pathology Laboratory, Faculty of Agriculture, Omar Al-Mukhtar University, and aimed to evaluate the antagonistic effect local isolate of Trichoderma harizianum against four pathogenic fungi (Botrytis cinerea, Macrophomina phaseolina, Rhizoctonia solani and Sclerotinia sclertiorum). Results showed that T. harzianum isolate had good significant ability to inhibit the studied diseases fungi directly or indirectly. Maximum rate of inhibition was 75.6%, and the highest degree of inhibition according 4 in Sookchaoy scale against M. phaseolina. The results of the microscopic examination showed the growth of the anti-fungus hyphae in parallel with the pathogenic hyphae and spirally wrapped around it, causing contraction and degradation them. Direct interaction between bio-control agent and the pathogens causing different diameters in halos lysis of pathogens, also, volatile and non-volatile substances that released by T. harzianum was recorded varying degrees of inhibition in all fungi, Finally, the bio-control agent produced antifungal compound that can inhibit the growth of all fungi 100%.
Keywords: Antagonism , Trichoderma harzianum, Phytopathogenic fungi.