Saif saadallah Hassan(1) and Abdullah Abdulkrim Hassan*(1)
(1). Department of Plant Protection, College of Agriculture, Tikrit University, Tikrit, Iraq.
(*Corresponding author: Abdullah Abdulkrim Hassan. Email: drabdullah.has67@tu.edu.iq).
Received: 02/05/2019 Accepted: 01/10/2019
Abstract
A laboratory studies were conducted during 2018/2019 season at Plant Protection Department, Faculty of Agriculture, University of Tikrit. The study investigated the bio-synthesis of the nanoparticles of an edible mushroom Pleurotus ostreatus A2019 using four fractions including fungus filtrate, biomass, hot extract and cold extract of the biomass. The results of the laboratory study indicated that the formation of silver nanoparticles from the Pleurotus eryngii,_by detecting the chromatic heterogeneity of these four fractions from yellow to brown, and recorded the highest absorption peaks (using the spectrophotometer) of the silver nanoparticles of all fractions within wavelengths ranged from 350 to 475 nm . All concentrations and fractions of prepared Ag nanoparticles showed the highest inhibition growth against pathogenic fungus P. aphanidermatum in comparison to control. The highest inhibition of linear growth was recorded at a concentration of 1.5 mM in the fungus filtrate treatment as it reached 0.66 mm, while the effect of concentration of 0.5 mM in the hot extract treatment of the biomass recorded the lowest inhibition linear growth 0.15 mm with significant differences between them. While the filtrate treatment showed significant superiority with the other treatments and it reached 0.42 mm compared to the other transactions, where the treatments of the cold and hot aqueous extract and biomass recorded 0.36, 0.16, 0.24 mm respectively.
Key words: Silver nanoparticles, Pleurotus spp, Pythum sp, Wheat seedlings.