Mai Moaalla*(1) Ahmad Ahmad(2) Omar Hammoudi(3) and Imad Daoud Ismaeil(3)
(1). Plant Protection Department, Faculty of Agriculture, Tishreen University, Latakia, Syria.
(2). Agriculture Research Center in Tartous, General Commission for Scientific Agricultural Research GCSAR, Damascus, Syria.
(3). Agriculture Research Center in Latakia, General Commission for Scientific Agricultural Research GCSAR, Damascus, Syria.
(*Corresponding author: Eng. Mai Moaalla. E-Mail: maimoalla92@gmail.com).
Received: 28/11/2019 Accepted: 31/12/2019
Abstract
This study was conducted to evaluate the effect of bacterial strain Bacillus subtillis FZB27 to induce systemic resistance against cucumber mosaic virus (CMV) in pepper plants using three kinds of applications (seeds, irrigation and seeds + irrigation). The study was carried out at the Scientific Agriculture Research Center in Tartus using randomized complete blocks design. Pepper seeds were submerged for 12 hours in suspension of Bacillus subtillis FZB27 with a concentration of 9×109 colony/ml formation unit. After one week after planting the seedlings were irrigated with 20 ml which was the same concentration suspension of Bacillus subtillis FZB27, then one week after transplanting, the plants were inoculated with C MV. The results showed that, there was a significant reducing in disease incidence of infected treated plants (50.72-57.16)% compared to the infected control (93.33)%, and the highest reduction was in (seeds+irrigation) application. There was also a significant reducing in disease severity of infected treated plants (48.2-60.17)% compared to the infected control (83.66)%, and the highest reduction was in (seeds+irrigation) application, with no significant differences among the treatment methods. The treatment with Bactria improved peroxidase enzyme activity, and consequently, there was significant increase in infected treated plants which ranged between (4.4-6.4) nmol compared to the infected control (2.5) nmol. The higher activity was with (seeds+irrigation) application. There was also significant increase in uninfected treated plants which ranged between (4.76-7.41) nmol compared to the healthy control (2.10) nmol. The higher activity was with (seeds+irrigation) application.
Key words: Induce systemic resistance, Cucumber mosaic virus, Bacillus subtillis FZB27, Pepper plants.