Sandy Douba*(1) Ahmed Shams ElDien Shaaban(2) Nawras AlAbras(3) and Yahya Kamari(4)
(1). Department. of Biotechnology, Faculty of Technological Engineering, Aleppo University, Aleppo, Syria.
(2). Department. of Biotechnology Engineering, Faculty of Technological Engineering, Aleppo University, Aleppo, Syria.
(3). National Commission of Biotechnology NCBT, Damascus, Syria.
(4). Aleppo Agricultural Research Center, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
(*Corresponding author: Eng. Sandy Douba. E-Mail: sandy_d14@hotmail.com).
Received: 28/10/2018 Accepted: 31/12/2018
Abstract
This study was carried out between March 2017 and May 2018. The leaves of Laurus nobilis L. were collected from Aleppo and Al-Nabk cities during flowering and fruiting stages. The essential oil was extracted using Clevenger and analyzed by gas chromatography and mass spectrometry (GC-MS). It was reported that the major compound in both oils was 1.8-cineole. Other predominant compounds of both oils were: Sabinene, α-Pinene, β-Pinene, Delta Careen. Antibacterial activity of the essential oil was tested against Gram-positive bacteria (Staphylococcus aureus) and three species of Gram-negative bacteria (Escherichia coli, Klepsiella pneumonia and Pseudomonas aeruginosa) using microdilution method. The concentrations of oil ranged from 1 to 100 μl/ml. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined. The oils extracted during flowering stage showed higher antibacterial activity than fruiting stage against most species of tested bacteria except Pseudomonas aeruginosa that was resistant to the higher concentrations that were used. The antibacterial activity of Aleppo oil was higher than Al-Nabk oil.
Keywords: Essential oil, Water extraction, Gram-positive bacteria, Chemical composition, Antibacterial activity, Bay laurel.
Full Paper in Arabic: PDF