Isolation and Identification of Bacillus mycoidis and Increase its Cellulolytic Efficiency by Using Acridine Orange Dye

SoadAbd Ali Atiya*(1) Labeeb Ahmad Kadhum(1) Sana Khuash Maid(1)  and ShahlaaKadhumFartoos(1)

(1). Directorate of Environment and Water, Ministry of Science and Technology, Baghdad, Iraq.

(*Corresponding author: SoadAbd Ali Atiya. E-Mail:

Received: 21/11/2018                                Accepted: 10/01/2019


This study was carried out at the Directorate of Environment and Water laboratories, Ministry of Science and Technology in Iraq. The study included collecting samples of agricultural wastes and isolation and identification of bacterial isolate from this waste by using the primary and secondary screening through morphological and biochemical testes that include api50. One isolate was identified belonging to Bacillus mycoidis in an additional test, the bacterial ability to lysis the cellulose through bacteria isolate growing on specific salt medium that contain cellulose was performed. Results showed a clear zone around the bacterial growth, which indicated bacterial ability in lysis of cellulose. The bacterial activity was under different concentrations of cellulose in a specific culture media. Results showed good bacterial growth at cellulose concentration of 0.6 % with optical density measured reaching 0.288.  Acridine orange effect on cellulolytic activity of bacterial isolate was studied. An increase of cellulolytic activity was found when the dye was used. Bacterial activity was detected by glucose concentration that measure the product of cellulose degradation, it reached 260 µg/ml in case the dye was used, while it was 230 µg/ml in case without dye. These results identified the suitability of cellulose concentration in culture media for the growth of Bacillus mycoidis (cellulose lysis bacteria), and the increase of cellulolytic activity by using acridine orange dye.

Key words: Cellulose, Bacillus mycoidis, Identification, Acridine orange.

Full Paper in Arabic: PDF