{"id":3252,"date":"2020-03-04T01:22:30","date_gmt":"2020-03-03T23:22:30","guid":{"rendered":"http:\/\/agri-research-journal.net\/?p=3252"},"modified":"2020-03-06T00:33:03","modified_gmt":"2020-03-05T22:33:03","slug":"%d8%aa%d8%b4%d8%ae%d9%8a%d8%b5-%d9%88%d9%83%d8%b4%d9%81-%d9%81%d9%8a%d8%b1%d9%88%d8%b3-%d8%a7%d9%84%d8%aa%d9%87%d8%a7%d8%a8-%d8%a7%d9%84%d9%82%d8%b5%d8%a8%d8%a7%d8%aa-%d8%a7%d9%84%d9%85%d8%b9%d8%af","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/sjar\/?p=3252","title":{"rendered":"\u062a\u0634\u062e\u064a\u0635 \u0648\u0643\u0634\u0641 \u0641\u064a\u0631\u0648\u0633 \u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0642\u0635\u0628\u0627\u062a \u0627\u0644\u0645\u0639\u062f\u064a \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 RT-PCR \u0641\u064a \u0645\u0632\u0627\u0631\u0639 \u062f\u062c\u0627\u062c \u0627\u0644\u0644\u062d\u0645 \u0641\u064a \u0633\u0648\u0631\u064a\u0629"},"content":{"rendered":"

\u062a\u0645\u0627\u0631\u0647 \u0623\u062d\u0645\u062f \u0627\u0644\u062c\u0644\u0627\u062f*(1)<\/sup> \u0641\u0647\u064a\u0645<\/strong> \u0639\u0644\u064a<\/strong> \u0639\u0628\u062f \u0627\u0644\u0639\u0632\u064a\u0632(2)<\/sup> \u0648\u0645\u062d\u0645\u062f \u062c\u0645\u064a\u0644 \u0633\u0644\u0647\u0628(3)<\/sup> \u0648\u0623\u0646\u0648\u0631 \u0645\u062d\u0645\u062f \u062f\u064a\u0628 \u0627\u0644\u0639\u0645\u0631(3)<\/sup><\/strong><\/p>\n

(1). \u0642\u0633\u0645 \u0627\u0644\u0625\u0646\u062a\u0627\u062c \u0627\u0644\u062d\u064a\u0648\u0627\u0646\u064a\u060c \u0643\u0644\u064a\u0629 \u0627\u0644\u0632\u0631\u0627\u0639\u0629\u060c \u062c\u0627\u0645\u0639\u0629 \u062a\u0634\u0631\u064a\u0646\u060c \u0633\u0648\u0631\u064a\u0629.<\/p>\n

(2). \u0642\u0633\u0645 \u0639\u0644\u0645 \u0627\u0644\u062d\u064a\u0627\u0629\u060c \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645\u060c \u062c\u0627\u0645\u0639\u0629 \u0637\u0631\u0637\u0648\u0633\u060c \u0637\u0631\u0637\u0648\u0633\u060c \u0633\u0648\u0631\u064a\u0629.<\/p>\n

(3). \u0645\u0631\u0643\u0632 \u0628\u062d\u0648\u062b \u0627\u0644\u0644\u0627\u0630\u0642\u064a\u0629\u060c \u0627\u0644\u0647\u064a\u0626\u0629 \u0627\u0644\u0639\u0627\u0645\u0629 \u0644\u0644\u0628\u062d\u0648\u062b \u0627\u0644\u0639\u0644\u0645\u064a\u0629 \u0627\u0644\u0632\u0631\u0627\u0639\u064a\u0629\u060c \u062f\u0645\u0634\u0642\u060c \u0633\u0648\u0631\u064a\u0629.<\/p>\n

(4). \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645\u060c \u062c\u0627\u0645\u0639\u0629 \u0627\u0644\u0628\u0639\u062b\u060c \u062d\u0645\u0635\u060c \u0633\u0648\u0631\u064a\u0629.<\/p>\n

(* \u0644\u0644\u0645\u0631\u0627\u0633\u0644\u0629: \u0645. \u062a\u0645\u0627\u0631\u0647 \u0623\u062d\u0645\u062f \u0627\u0644\u062c\u0644\u0627\u062f. \u0627\u0644\u0628\u0631\u064a\u062f \u0627\u0644\u0625\u0644\u0643\u062a\u0631\u0648\u0646\u064a: tamara.aljallad@gmail.com<\/a>).<\/p>\n

\u062a\u0627\u0631\u064a\u062e \u0627\u0644\u0627\u0633\u062a\u0644\u0627\u0645: 26\/08\/2019\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u062a\u0627\u0631\u064a\u062e \u0627\u0644\u0642\u0628\u0648\u0644:\u00a0 02\/10\/2019<\/p>\n

\u0627\u0644\u0645\u0644\u062e\u0635<\/strong><\/p>\n

\u0627\u0633\u062a\u064f\u062e\u062f\u0645\u062a \u062a\u0642\u0627\u0646\u0629 \u062a\u0641\u0627\u0639\u0644 \u0627\u0644\u0628\u0648\u0644\u064a\u0645\u064a\u0631\u0627\u0632 \u0627\u0644\u0645\u062a\u0633\u0644\u0633\u0644 \u0627\u0644\u0639\u0643\u0633\u064a (reverse transcriptase-polymerase chain reaction (RT-PCR \u0644\u0644\u0643\u0634\u0641 \u0639\u0646 \u0641\u064a\u0631\u0648\u0633 \u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0642\u0635\u0628\u0627\u062a \u0627\u0644\u0645\u0639\u062f\u064a IBV) infectious bronchitis virus) \u0641\u064a \u0642\u0637\u0639\u0627\u0646 \u0627\u0644\u0641\u0631\u0648\u062c \u0627\u0644\u062a\u062c\u0627\u0631\u064a\u0629 \u0641\u064a \u0633\u0648\u0631\u064a\u0629\u060c \u062d\u064a\u062b \u062c\u0645\u0639\u062a 50 \u0639\u064a\u0646\u0629 \u0645\u0631\u0636\u064a\u0629 \u0645\u0634\u062a\u0628\u0647\u0629 \u0645\u0646 \u0623\u0646\u0633\u062c\u0629 \u0627\u0644\u0631\u063a\u0627\u0645\u0649 \u0641\u064a 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\u0627\u0644\u062d\u0645\u0636 \u0627\u0644\u0646\u0648\u0648\u064a \u0627\u0644\u0641\u064a\u0631\u0648\u0633\u064a\u00a0 RNA \u0645\u0646 \u0623\u0646\u0633\u062c\u0629 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0631\u0636\u064a\u0629 \u0645\u0628\u0627\u0634\u0631\u0629\u060c \u0648\u0645\u0646 \u062b\u0645 \u062a\u062d\u0648\u064a\u0644 RNA \u00a0\u0625\u0644\u0649 cDNA \u0628\u062a\u0642\u0646\u064a\u0629 \u0627\u0644\u0646\u0633\u062e \u0627\u0644\u0639\u0643\u0633\u064a RT-PCR\u060c \u0648\u0627\u062e\u0636\u0627\u0639\u0647\u0627 \u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u062a\u0641\u0627\u0639\u0644 PCR\u060c \u0648\u062a\u0641\u0627\u0639\u0644 Nested PCR. \u0627\u062e\u062a\u064a\u0631\u062a \u0627\u0644\u0628\u0627\u062f\u0626\u0627\u062a \u0627\u0644\u0645\u0633\u062a\u062e\u062f\u0645\u0629 \u0641\u064a \u062a\u0641\u0627\u0639\u0644 RT-PCR \u0645\u0646 \u062a\u062d\u062a \u0648\u062d\u062f\u0629 S1 \u0644\u0644\u0645\u0648\u0631\u062b\u0629 S) spike) \u0627\u0644\u062a\u064a \u062a\u062a\u0631\u0643\u0632 \u0641\u064a\u0647\u0627 \u0627\u0644\u0637\u0641\u0631\u0627\u062a \u0627\u0644\u062a\u064a \u062a\u0635\u064a\u0628 \u0645\u0648\u0631\u062b\u0629 \u0627\u0644\u0641\u064a\u0631\u0648\u0633 \u0641\u064a \u0647\u0630\u0647 \u0627\u0644\u0645\u0646\u0637\u0642\u0629 (\u0645\u0646\u0637\u0642\u0629 \u0634\u062f\u064a\u062f\u0629 \u0627\u0644\u062a\u0628\u0627\u064a\u0646)\u060c \u0643\u0645\u0627 \u062a\u0645 \u062a\u0645\u0631\u064a\u0631 10 \u0645\u0646 \u0627\u0644\u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0625\u064a\u062c\u0627\u0628\u064a\u0629 \u0627\u0644\u0645\u062d\u062a\u0648\u064a\u0629 \u0639\u0644\u0649 \u0627\u0644\u0641\u064a\u0631\u0648\u0633 \u0628\u0623\u062c\u0646\u0629 \u0627\u0644\u062f\u062c\u0627\u062c \u0627\u0644\u0646\u0627\u0645\u064a\u0629 \u0627\u0644\u062e\u0627\u0644\u064a\u0629 \u0645\u0646 \u0627\u0644\u0639\u0648\u0627\u0645\u0644 \u0627\u0644\u0645\u0645\u0631\u0636\u0629\u060c Specific pathogen free embryonated egg \u00a0((SPF \u0628\u0639\u0645\u0631 9-11\u00a0 \u064a\u0648\u0645 \u062a\u062d\u0636\u064a\u0646 \u0648\u062d\u0633\u0628 \u0627\u0644\u0637\u0631\u0642 \u0627\u0644\u0645\u0639\u062a\u0645\u062f\u0629 \u0641\u064a \u0639\u0644\u0645 \u0627\u0644\u0641\u064a\u0631\u0648\u0633\u0627\u062a. \u0646\u064f\u0641\u0630 \u0627\u0644\u0628\u062d\u062b \u0641\u064a \u0645\u062e\u0627\u0628\u0631 \u0627\u0644\u0647\u064a\u0626\u0629 \u0627\u0644\u0639\u0627\u0645\u0629 \u0644\u0644\u0628\u062d\u0648\u062b \u0627\u0644\u0639\u0644\u0645\u064a\u0629 \u0627\u0644\u0632\u0631\u0627\u0639\u064a\u0629 (\u0645\u0631\u0643\u0632 \u0628\u062d\u0648\u062b \u0627\u0644\u0644\u0627\u0630\u0642\u064a\u0629) \u0628\u0627\u0644\u062a\u0639\u0627\u0648\u0646 \u0645\u0639 \u0645\u062e\u0628\u0631 PCR \u00a0\u0641\u064a \u0643\u0644\u064a\u0629 \u0627\u0644\u0637\u0628 \u0627\u0644\u0628\u064a\u0637\u0631\u064a \u0628\u062d\u0645\u0627\u0647. \u0628\u064a\u0651\u0646\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u062e\u062a\u0628\u0627\u0631 RT-PCR\u060c \u0648\u062c\u0648\u062f 37 \u062d\u0627\u0644\u0629 \u0625\u064a\u062c\u0627\u0628\u064a\u0629 \u0644\u0644\u0627\u062e\u062a\u0628\u0627\u0631 \u0648\u0628\u0646\u0633\u0628\u0629 74% \u0645\u0646 \u0639\u064a\u0646\u0627\u062a \u0627\u0644\u0645\u0632\u0627\u0631\u0639 \u0627\u0644\u0645\u062e\u062a\u0628\u0631\u0629\u060c \u0648\u0623\u0638\u0647\u0631\u062a \u0646\u062a\u0627\u0626\u062c \u0627\u0644\u062d\u0642\u0646 \u0641\u064a \u0623\u062c\u0646\u0629 \u0627\u0644\u062f\u062c\u0627\u062c \u0627\u0644\u0646\u0627\u0645\u064a\u0629 \u0622\u0641\u0627\u062a \u062a\u0634\u0631\u064a\u062d\u064a\u0629 \u0648\u0627\u0636\u062d\u0629 \u0648\u0645\u0645\u064a\u0632\u0629\u00a0 \u0644\u0641\u064a\u0631\u0648\u0633 \u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0642\u0635\u0628\u0627\u062a \u0627\u0644\u0645\u0639\u062f\u064a \u0628\u0639\u062f 5- 6 \u0623\u064a\u0627\u0645 \u0645\u0646 \u0627\u0644\u062d\u0642\u0646\u060c \u0648\u062a\u0623\u062e\u0631 \u0648\u0639\u062f\u0645 \u062a\u0637\u0648\u0631 \u0648\u0646\u0645\u0648 \u0627\u0644\u062c\u0646\u064a\u0646 (\u062a\u0642\u0632\u0645)\u060c \u0648\u062a\u0634\u0627\u0628\u0643 \u0623\u0635\u0627\u0628\u0639 \u0648\u0646\u0632\u0641 \u062f\u0645\u0648\u064a\u060c \u0628\u0627\u0644\u0645\u0642\u0627\u0631\u0646\u0629 \u0645\u0639 \u0627\u0644\u0634\u0627\u0647\u062f \u0627\u0644\u0633\u0644\u0628\u064a\u060c \u0648\u0623\u0638\u0647\u0631\u062a \u0627\u0644\u0646\u062a\u0627\u0626\u062c \u0623\u064a\u0636\u0627\u064b \u062d\u0633\u0627\u0633\u064a\u0629\u00a0 \u062a\u0642\u0646\u064a\u0629 RT-PCR \u0644\u0644\u0643\u0634\u0641 \u0639\u0646 \u0641\u064a\u0631\u0648\u0633 IBV.<\/p>\n

\u0627\u0644\u0643\u0644\u0645\u0627\u062a \u0627\u0644\u0645\u0641\u062a\u0627\u062d\u064a\u0629:<\/strong> \u0641\u064a\u0631\u0648\u0633 \u0627\u0644\u062a\u0647\u0627\u0628 \u0627\u0644\u0642\u0635\u0628\u0627\u062a \u0627\u0644\u0645\u0639\u062f\u064a IBV\u060c RT-PCR\u060c \u062f\u062c\u0627\u062c \u0627\u0644\u0644\u062d\u0645.<\/p>\n

\u0627\u0644\u0628\u062d\u062b \u0643\u0627\u0645\u0644\u0627\u064b \u0628\u0627\u0644\u0644\u063a\u0629 \u0627\u0644\u0639\u0631\u0628\u064a\u0629: PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u062a\u0645\u0627\u0631\u0647 \u0623\u062d\u0645\u062f \u0627\u0644\u062c\u0644\u0627\u062f*(1) \u0641\u0647\u064a\u0645 \u0639\u0644\u064a \u0639\u0628\u062f \u0627\u0644\u0639\u0632\u064a\u0632(2) \u0648\u0645\u062d\u0645\u062f \u062c\u0645\u064a\u0644 \u0633\u0644\u0647\u0628(3) \u0648\u0623\u0646\u0648\u0631 \u0645\u062d\u0645\u062f \u062f\u064a\u0628 \u0627\u0644\u0639\u0645\u0631(3) (1). \u0642\u0633\u0645 \u0627\u0644\u0625\u0646\u062a\u0627\u062c \u0627\u0644\u062d\u064a\u0648\u0627\u0646\u064a\u060c \u0643\u0644\u064a\u0629 \u0627\u0644\u0632\u0631\u0627\u0639\u0629\u060c \u062c\u0627\u0645\u0639\u0629 \u062a\u0634\u0631\u064a\u0646\u060c \u0633\u0648\u0631\u064a\u0629. (2). \u0642\u0633\u0645 \u0639\u0644\u0645 \u0627\u0644\u062d\u064a\u0627\u0629\u060c \u0643\u0644\u064a\u0629 \u0627\u0644\u0639\u0644\u0648\u0645\u060c \u062c\u0627\u0645\u0639\u0629 \u0637\u0631\u0637\u0648\u0633\u060c \u0637\u0631\u0637\u0648\u0633\u060c \u0633\u0648\u0631\u064a\u0629. (3). \u0645\u0631\u0643\u0632 \u0628\u062d\u0648\u062b \u0627\u0644\u0644\u0627\u0630\u0642\u064a\u0629\u060c \u0627\u0644\u0647\u064a\u0626\u0629 \u0627\u0644\u0639\u0627\u0645\u0629 \u0644\u0644\u0628\u062d\u0648\u062b … \u0623\u0643\u0645\u0644 \u0642\u0631\u0627\u0621\u0629 \u0627\u0644\u0645\u0642\u0627\u0644\u0629 ←<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1263,125],"tags":[1270,1271,1269],"class_list":["post-3252","post","type-post","status-publish","format-standard","hentry","category--7--1-2020","category-125","tag-rt-pcr","tag-1271","tag-----ibv"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/posts\/3252","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3252"}],"version-history":[{"count":2,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/posts\/3252\/revisions"}],"predecessor-version":[{"id":3384,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=\/wp\/v2\/posts\/3252\/revisions\/3384"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3252"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3252"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/sjar\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}