{"id":9806,"date":"2025-08-24T19:31:14","date_gmt":"2025-08-24T19:31:14","guid":{"rendered":"https:\/\/agri-research-journal.net\/SjarEn\/?p=9806"},"modified":"2025-08-24T19:31:14","modified_gmt":"2025-08-24T19:31:14","slug":"the-effect-of-different-concentrations-of-24-d-and-naa-on-callus-induction-on-different-plant-explants-of-limon","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=9806","title":{"rendered":"The Effect of Different Concentrations of 2,4-D and NAA on Callus Induction on Different Plant Explants of limon"},"content":{"rendered":"

Sedra Al-Kaddour<\/strong>(1)<\/sup><\/strong>, Ahmad Shams Al-Dien Shaaban<\/strong>(1)<\/sup><\/strong>*<\/sup><\/strong> , and Abd Al-Kareem Barakat<\/strong>(2)<\/sup><\/strong><\/span><\/p>\n

(1).\u00a0 Dept of Biotechnology, Faculty of Technical Engineering, University of Aleppo, Syria<\/span><\/p>\n

(2). Dept of Horticulture, Faculty of Agricultural Engineering, University of Aleppo, Syria.<\/span><\/p>\n

(*Corresponding author: Ahmad Shams Al-Dien Shaaban E-Mail: shaabany57@gmail.com<\/a> ).<\/u><\/span><\/p>\n

Received:4\/03\/2024\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/strong>Accepted:21\/04\/2024<\/span><\/p>\n

Abstract<\/strong><\/span><\/p>\n

Factorial experiments were conducted out aimed to determine the optimal medium for callus induction and was conducted between 1\/4\/2023 and 20\/10\/2023 at the Plant Tissue Culture Laboratory of the Faculty of Agricultural Engineering and the laboratories of the Faculty of Technical Engineering at Aleppo University. Different plant parts (leaf, internode, Hypocotyl) were planted in MS medium using a constant concentration of BAP. The media was treated with different concentrations of plant growth regulators 2,4-D and NAA (1, 2, 3, 4, 8, 12 mg\/L). The experiment conducted using a completely randomized design with 3 replicates, The results showed that the rate of callus generation at the growth 2,4-D was the highest (100%) using low concentrations (1, 2, 3 mg\/L) at the three plant parts, taking consideration account the growth rate of callus, and calluses generation at the growth regulator NAA was higher (100%) using medium concentrations (4 mg\/L) at the embryonic peduncle and the highest concentration (12 mg\/L) at all plant parts. The best combination was at Hypocotyl and 8 mg\/l of NAA, followed by internode and 8 mg\/L of 2.4-D, the average weight of callus was (0.583, 0.503 g), respectively. i.e. 8 mg\/l is best at both regulators.<\/span><\/p>\n

Keywords<\/strong>: 2,4-D, NAA, Leaf, Hypocotyle, Internode, Callus.<\/span><\/p>\n

Full paper in Arabic: PDF<\/a><\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Sedra Al-Kaddour(1), Ahmad Shams Al-Dien Shaaban(1)* , and Abd Al-Kareem Barakat(2) (1).\u00a0 Dept of Biotechnology, Faculty of Technical Engineering, University of Aleppo, Syria (2). Dept of Horticulture, Faculty of Agricultural Engineering, University of Aleppo, Syria. (*Corresponding author: Ahmad Shams Al-Dien … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-9806","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9806","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9806"}],"version-history":[{"count":1,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9806\/revisions"}],"predecessor-version":[{"id":9811,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9806\/revisions\/9811"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9806"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}