{"id":9796,"date":"2025-08-24T19:18:35","date_gmt":"2025-08-24T19:18:35","guid":{"rendered":"https:\/\/agri-research-journal.net\/SjarEn\/?p=9796"},"modified":"2025-08-24T19:18:35","modified_gmt":"2025-08-24T19:18:35","slug":"studying-the-content-of-phenols-and-flavonoids-of-thuja-orientalis-leaf-extracts-and-evaluating-their-antioxidant-and-antibacterial-activities","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=9796","title":{"rendered":"Studying the content of phenols and flavonoids of\u00a0 Thuja orientalis leaf extracts, and evaluating their antioxidant and antibacterial activities"},"content":{"rendered":"

\u00a0<\/strong>Bassam Al-Oklah * (1)<\/sup> <\/strong>Jalal Fandi (2) <\/sup>\u00a0Rudwan Badr Al-Deen (<\/sup><\/strong>1<\/sup><\/strong>) \u00a0<\/sup><\/strong>
\nNour Haj Masoud <\/strong>(<\/sup><\/strong>1<\/sup><\/strong>) <\/sup><\/strong>Lamyaa Husamo <\/strong>(<\/sup><\/strong>1<\/sup><\/strong>)<\/sup><\/strong><\/span><\/p>\n

(1). National Commission for Biotechnology, Damascus, Syria.\u00a0<\/span><\/p>\n

(2). Faculty of Pharmacy, Damascus University, Damascus, Syria.<\/span><\/p>\n

(*Corresponding author: Dr. Bassam Al-Oklah, E-Mail: bassam780alk@gmail.com<\/a>, phone: 0938994115).<\/span><\/p>\n

Received: 1\/05\/2023\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Accepted: 9\/03\/2024<\/span><\/p>\n

Abstract:\u00a0<\/strong><\/span><\/p>\n

This paper aimed to prepare efficient extracts from Thuja orientalis<\/em> plant leaves by using several solvents (ethyl acetate, methanol, acetone), and study their total phenolic and flavonoid content, their antioxidant activity, and antibacterial activity against gram positive bacteria (Staphylococcus saprophyticus<\/em>, <\/em>Staphylococcus aureu<\/em>s, <\/em>Staphylococcus homini<\/em>s<\/em>) and gram negative bacteria (Enterobacter cloacae<\/em>, Klebsiella oxytoca<\/em>, E.coli<\/em>). Their phenol and flavonoid contents were evaluated by\u00a0 Folin-Ciocalteu regent method and aluminum chloride method respectively. Their antioxidant activities were determined by estimating\u00a0 scavenging capacity towards DPPH free radical. And their antibacterial activities were estimated by disc diffusion method. The results showed that the leaf extract prepared from acetone reached the highest content of\u00a0 phenols (26000 mg\/100g) and flavonoids (295mg\/100g), and the highest activity of scavenging DPPH free radical (75%). The extracts of Thuja orientalis<\/em> plant leaves showed high antibacterial activities against gram negative bacteria comparing with gram positive ones, and the highest antibacterial activity was against Klebsiella oxytoca<\/em>.<\/span><\/p>\n

Keywords:<\/strong> Thuja orientalis<\/em>, antibacterial activity, antioxidant activity, phenols, flavonoids.<\/span><\/p>\n

Full paper in Arabic<\/span><\/strong>:<\/span><\/strong> PDF<\/span><\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u00a0Bassam Al-Oklah * (1) Jalal Fandi (2) \u00a0Rudwan Badr Al-Deen (1) \u00a0 Nour Haj Masoud (1) Lamyaa Husamo (1) (1). National Commission for Biotechnology, Damascus, Syria.\u00a0 (2). Faculty of Pharmacy, Damascus University, Damascus, Syria. (*Corresponding author: Dr. Bassam Al-Oklah, E-Mail: … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-9796","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9796","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9796"}],"version-history":[{"count":1,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9796\/revisions"}],"predecessor-version":[{"id":9799,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/9796\/revisions\/9799"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9796"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9796"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9796"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}