{"id":7544,"date":"2024-02-24T17:45:24","date_gmt":"2024-02-24T17:45:24","guid":{"rendered":"https:\/\/agri-research-journal.net\/SjarEn\/?p=7544"},"modified":"2024-02-24T17:45:53","modified_gmt":"2024-02-24T17:45:53","slug":"detection-of-genetic-variation-in-mutants-potato-clones-using-rapd-primers","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=7544","title":{"rendered":"Detection of Genetic Variation in Mutants Potato Clones Using RAPD Primers"},"content":{"rendered":"

Wael Mtawj <\/strong>(<\/sup><\/strong>1<\/sup><\/strong> )<\/sup><\/strong>*, <\/strong>Mazen Rajab(1)<\/sup>and Hassan Khojah(2)<\/sup><\/strong><\/span><\/p>\n

(1). Researcher, General Commission for Scientific Agricultural Research (GCSAR), Biotechnology Dept, Lattakia, Syria<\/span><\/p>\n

(2). Professor, Horticulture Dept., Faculty of Agriculture, Tishreen, University, Lattakia, Syria.<\/span><\/p>\n

(*Corresponding author: Wael Mtawj. E-Mail.:mtwjmrym@gmail.com<\/a>)<\/u><\/span><\/p>\n

Received: 14\/06\/2022\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Accepted: 25\/10\/2022<\/span><\/p>\n

Abstract:\u00a0<\/strong><\/span><\/p>\n

This study was conducted at Lattakia Research Center, General Commission for Scientific Agricultural Research GCSAR, during 2017\/2016. The random amplified polymorphic DNA (RAPD) based on Polymerase Chain Reaction (PCR) with fifteen primers were applied, used to estimate genetics variation to six clones chemical mutagen by EMS (Ethel methan sulphonat) and salt tolerant M5 of potato Marfona cv. umutant. The results showed that the treatment 40mM\/2-3hr were the bes and tthe clones 403P14, 403P2, 402P31 were the most tolerant for salinity. Phases of work included the isolation and purification of DNA plant parts and DNA polymorphisms were scored within amplified fragements by electrophoresis. The results of RAPD analysis were clear differences in the number of DNA amplified fragments and molecular weights depending on the primers user of the 15 random primers tested, 3 primers generate polymorphic RAPD patterns. These primers produced 27 bands\u00a0 across all 6 potato clones corresponding to average of 9.0 bands per primer. Only 6 bands of them were monomorphic, A total number of polymorphic amplified 21 fragments for all primers used. whereas the others were polymorphic reaching 77.03 % of the total bands produced. The maximum number of bands was 13 with opj01 primer tested, while the lowest number of bands (6) observed with the primer OPC04. The results were showed than potato clones (403p2, 403p14,404p13) variation on Marfona cv. umutant by number of polymorphic amplified (5,10,12) fragments, while the lowest number of Polymorphic (1,1,2) bands of the clones (402p31,403p42,404p46) for all primers used.<\/span><\/p>\n

Keywords<\/strong>: Potato , Marfona , RAPD.<\/span><\/p>\n

Full paper in Arabic:\u00a0 pdf<\/a><\/span><\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Wael Mtawj (1 )*, Mazen Rajab(1)and Hassan Khojah(2) (1). Researcher, General Commission for Scientific Agricultural Research (GCSAR), Biotechnology Dept, Lattakia, Syria (2). Professor, Horticulture Dept., Faculty of Agriculture, Tishreen, University, Lattakia, Syria. (*Corresponding author: Wael Mtawj. E-Mail.:mtwjmrym@gmail.com) Received: 14\/06\/2022\u00a0 \u00a0 … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7544","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7544","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7544"}],"version-history":[{"count":2,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7544\/revisions"}],"predecessor-version":[{"id":7547,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7544\/revisions\/7547"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7544"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7544"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}