{"id":7345,"date":"2023-12-15T13:56:55","date_gmt":"2023-12-15T13:56:55","guid":{"rendered":"https:\/\/agri-research-journal.net\/SjarEn\/?p=7345"},"modified":"2023-12-15T13:57:30","modified_gmt":"2023-12-15T13:57:30","slug":"effects-of-magnetic-field-m-f-in-milk-thistle-silybum-marianum-l-callus-cultures-induction-and-accumulation-of-silybin-by-using-hplc-technique","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=7345","title":{"rendered":"Effects of Magnetic Field (M.F.) in Milk Thistle (Silybum marianum L.) Callus Cultures Induction and Accumulation of Silybin by Using (HPLC) Technique"},"content":{"rendered":"

Sabah A. Al-Badrani \u00a0(<\/sup><\/strong>1<\/sup><\/strong>)<\/sup><\/strong> and Rehab A. H. AL-Baker (2) <\/sup>*<\/strong><\/span><\/p>\n

(1). Qbat Al-Saskra School for Girls, General Directorate of Nineveh Education, Iraq.<\/span><\/p>\n

(2). Dept. of Environmental Sciences\/ College of Environmental Sciences and Technologies\/University of Mosul, Iraq.<\/span><\/p>\n

(*Corresponding author: Rehab A. H. AL-Baker. E-Mail: rehsbio39@uomosul.edu.iq<\/a>).<\/span><\/p>\n

Received: 1\/02\/2022\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 14\/10\/2022<\/span><\/p>\n

Abstract:\u00a0<\/strong><\/span><\/p>\n

The research was carried out in laboratories of the college science, University of Mosul between (2019-2020). The study aimed to detect the magnetic field (M.F.) activity in initiation and growth of Silybum<\/em> marianum <\/em>L. callus cultures from sterilized seedlings segments (roots, leaves, stems and nodes) which had been cultured on solid (MS) medium supplemented with (3.0)mg\/L of Benzyl adenine (BA) and (5.0) mg\/L of Naphthalene Acetic Acid(NAA). Besides measuring different callus biomarkers, and explaining the role of the (M.F.) in silybin accumulation in callus cultures, based on High Performance Liquid Chromatography (HPLC) Technique. The results indicated that the best response was for the roots callus cultures compared with the rest of the cultures used in the research, as the increase in fresh weight of roots callus after expose to magnetic field (M.F.), which reached (4.0) g fresh weight and (96%) viability, (1.85) mg\/g protein concentration after 40 days. HPLC results revealed a dramatic increase in accumulation of active compound (silybin) by M.F. for all callus cultures depending on Retention time (Rt<\/sub>) and area under the curve for the standard sample (ST).<\/span><\/p>\n

Keywords: <\/strong>(HPLC) Technique, Magnetic Field (M.F.), Silybin, Silybum marianum<\/em> callus.<\/span><\/p>\n

Full paper in English:\u00a0 pdf<\/a><\/span><\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Sabah A. Al-Badrani \u00a0(1) and Rehab A. H. AL-Baker (2) * (1). Qbat Al-Saskra School for Girls, General Directorate of Nineveh Education, Iraq. (2). Dept. of Environmental Sciences\/ College of Environmental Sciences and Technologies\/University of Mosul, Iraq. (*Corresponding author: Rehab … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-7345","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7345","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=7345"}],"version-history":[{"count":2,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7345\/revisions"}],"predecessor-version":[{"id":7348,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/7345\/revisions\/7348"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=7345"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=7345"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=7345"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}