{"id":6870,"date":"2023-08-20T09:31:34","date_gmt":"2023-08-20T09:31:34","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=6870"},"modified":"2023-08-20T09:31:34","modified_gmt":"2023-08-20T09:31:34","slug":"determination-of-genetic-diversity-of-shamii-cattle-using-issr-pcr","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=6870","title":{"rendered":"Determination of genetic diversity of Shamii cattle using ISSR-PCR"},"content":{"rendered":"

Hassan Ahmed Muhdi<\/strong> (<\/sup><\/strong>1)*<\/sup><\/strong>, Ismaeel Al-Saleh(1)<\/sup>, Mansour Ahmed(2)<\/sup>,  and Al-Mu\u2019tasim Billah Al-Daqer(2)<\/sup><\/strong><\/span><\/p>\n

(1). Department of Medical and Animal Biotechnology, National Commission for Biotechnology, Ministry of Higher Education and Scientific Research, Damascus, Syria.<\/span><\/p>\n

(2). General Commission for Scientific Agricultural Research \u2013 Animal Wealth for Research Administration, Damascus, Syria.<\/span><\/p>\n

(*Corresponding Author: Dr. Hassan Muhdi, E-mail: Dr74hassan@gmail.com<\/a>).<\/span><\/p>\n

Received:26\/02\/2022       Acceptance: 5\/06\/2022<\/span><\/p>\n

Abstract: <\/strong><\/span><\/p>\n

This study was conducted to determine the genetic diversity of Shamii cattle compared to Friesian, Using the Intra simple sequencing repeats (ISSR) by the primer (AGC)6C. Blood samples were drawn from 13 cows (9 Shamii and 4 Friesian). DNA Isolated from blood. PCR products showed 27 different bands with lengths ranging from 158 to 1030 bp in the two strains. Parameters of genetic variance, including the effective number of alleles, Shannon index, and genetic diversity were estimated and the genetic matrix was calculated. Dendogram was drawn. The Shannon coefficient (I-index) values \u200b\u200branged between (0-0.69), with a mean of 0.54, and genetic diversity (h) ranged between (0-0.5) and with a mean of 0.36, as for the number of active alleles (ne) it ranged between (1- 1.988), with a mean of 1.62. The value of the average polymorphic informatics content (PIC) for Shami cows was (0.4479) and for Friesian cows (0.4966). The results of the genetic matrix and dendrogram showed that (AGC)6C  microsatellite is reliable and can be used in the study of genetic diversity; Where it separated the two breeds of Shami cows and Friesian cows.<\/span><\/p>\n

Keywords:<\/strong> Shamii cattle, ISSR, (AGC)6C, genetic fingerprinting<\/span><\/p>\n

Full paper in Arabic: pdf<\/a><\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Hassan Ahmed Muhdi (1)*, Ismaeel Al-Saleh(1), Mansour Ahmed(2),  and Al-Mu\u2019tasim Billah Al-Daqer(2) (1). Department of Medical and Animal Biotechnology, National Commission for Biotechnology, Ministry of Higher Education and Scientific Research, Damascus, Syria. (2). General Commission for Scientific Agricultural Research \u2013 … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6870","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6870","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6870"}],"version-history":[{"count":1,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6870\/revisions"}],"predecessor-version":[{"id":6874,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6870\/revisions\/6874"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6870"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6870"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6870"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}