{"id":6442,"date":"2023-04-17T20:34:13","date_gmt":"2023-04-17T20:34:13","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=6442"},"modified":"2023-04-17T20:35:32","modified_gmt":"2023-04-17T20:35:32","slug":"enhancing-an-in-vitro-shoot-formation-of-ixora-coccinea-l-using-silver-nitrate","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=6442","title":{"rendered":"Enhancing an In Vitro Shoot Formation of Ixora coccinea L.  Using Silver Nitrate"},"content":{"rendered":"<p style=\"text-align: center;\"><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\"><strong>Rania Ahmed Hassan Onsa<sup>(1)*<\/sup>, Igbal Abd Elgadeir Abd Ellatif <sup>(2)<\/sup>, Magdoleen Gamar Eldeen Osman<sup>(3)<\/sup><\/strong><\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 16px;\">(1). Department of Horticulture, Faculty of Agriculture, University of Al zaiem Al azhari , Khartoum, Sudan<\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 16px;\">(2). Department of Horticulture, Faculty of Agriculture Studies, Sudan University of Science and Technology, Khartoum, Sudan<\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 16px;\">(3). Plant Tissue Culture, National Center of Research, Khartoum, Sudan.<\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 16px;\"><strong><sup>(*<\/sup><\/strong>Corresponding author: Dr. Rania Ahmed Hassan Onsa, E-mail: <a href=\"mailto:raniaonsa@gmail.com\">raniaonsa@gmail.com<\/a><u>)<\/u><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\">\u00a0\u00a0\u00a0\u00a0\u00a0<span style=\"font-size: 16px;\">\u00a0 Received: 16\/12\/ 2021\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 16\/03\/ 2022<\/span><\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\"><strong>Abstract:\u00a0<\/strong><\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\">Ixora coccinea is a popular ornamental plant, as well as it is an important medicinal plant. This study was conducted to study the effect of silver nitrate (AgNO<sub>3<\/sub>) on shoot formation of <em>Ixora coccinea<\/em>. Shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.0, 2.5, 5.0, 8.0 and 10.0 mg\/l) of AgNO<sub>3<\/sub> for establishment stage. Then shoot tip explants sub-cultured on MS medium supplemented with 8.0 mg \/l AgNO<sub>3<\/sub> combined with different concentrations (0.0,0.5,1.0 and 2.0 mg\/l) of Benzyl aminopurine (BAP) for multiplication stage. The results showed that uses of different concentrations of AgNO<sub>3<\/sub> alone affect positively on shoot morphogenesis of <em>I. coccinea<\/em>. 8.0 mg \/l of AgNO<sub>3<\/sub> gave the best result on shoot formation. However, 5.0, 8.0 and 10.0 mg\/l AgNO<sub>3<\/sub> scored similar results in number of nodes. The combine of 8.0 mg \/l AgNO<sub>3<\/sub> and 2.0 mg\/l BAP to MS medium enhanced the multiplication rate of shoot significantly.<\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\"><strong>Keywords<\/strong>: AgNO<sub>3<\/sub>, <em>In vitro<\/em>, <em>Ixora coccinea<\/em>, Micropropagation, Shoot formation.<\/span><\/p>\n<p><span style=\"font-family: 'Times New Roman', Times; font-size: 17px;\">Full paper in English: <a href=\"http:\/\/agri-research-journal.net\/SjarEn\/wp-content\/uploads\/v10n2p6.pdf\">pdf<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Rania Ahmed Hassan Onsa(1)*, Igbal Abd Elgadeir Abd Ellatif (2), Magdoleen Gamar Eldeen Osman(3) (1). Department of Horticulture, Faculty of Agriculture, University of Al zaiem Al azhari , Khartoum, Sudan (2). Department of Horticulture, Faculty of Agriculture Studies, Sudan University &hellip; <a href=\"https:\/\/agri-research-journal.net\/SjarEn\/?p=6442\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-6442","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6442","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6442"}],"version-history":[{"count":2,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6442\/revisions"}],"predecessor-version":[{"id":6448,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/6442\/revisions\/6448"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6442"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6442"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6442"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}