{"id":477,"date":"2016-05-08T02:50:05","date_gmt":"2016-05-08T02:50:05","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=477"},"modified":"2016-11-19T10:13:25","modified_gmt":"2016-11-19T10:13:25","slug":"vol3no1p2","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=477","title":{"rendered":"Molecular Study of Pear Psylla Cacopsylla<\/em> spp. (Psyllidae: \u00a0Hemiptera) in Middle and Southern Regions of Syria"},"content":{"rendered":"

Bassam Oudeh*(1)<\/sup>\u00a0 Wajih Kassis (2)<\/sup> and Randa Abu-Tara (3)<\/sup><\/strong><\/p>\n

(1). Mokhtaria Research Station, Agricultural Scientific Research Center at Homs, General Commission for Scientific Agricultural Research (GCSAR), Homs, Syria.
\n(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria.
\n(3). Faculty of Science, Damascus University, Damascus, Syria.<\/p>\n

(*Corresponding author: Dr. Bassam Oudeh. E-Mail: B_oudeh@hotmail.com<\/a>).<\/p>\n

Received: 01\/02\/ 2015\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 31\/07\/ 2015<\/p>\n

Abstract<\/strong><\/p>\n

Molecular study of pear psylla Cacopsylla <\/em>spp. (Psylldae: Hemiptera) was carried out in the biotechnology laboratory, Faculty of Agriculture, Damascus University during the growing season 2013\/ 2014. Samples were collected from four governorates located in middle and southern regions of Syria: Homs (Mokhtaria Research Station and Al- Rastan), Hama (Tezeen), Damascus rural (Al-Zabadany) and Al-Sweida (Al-Sweida Research Center). The results showed that genetic differences of pear psylla by using ISSR with 19 primers, only 12 primers were succeeded in amplifying the male \u0301s DNA of pear psylla. The total number of bands was 70, only 64 bands were polymorphic, the percentage of polymorphism between males was (93.06%), males of pear psylla of Damascus rural and Al-Sweida were the most closer (86.12%), the cluster analysis separated the males of\u00a0 Hama in an independent first group, and the second was separated into two sub clusters, males of Al-Rastan and Mokhtaria Research Station were included in the first sub clusters,\u00a0 males of Damascus rural and Al-Sweida were included in the second. Only 15 primers were succeeded in amplifying the female \u0301s DNA of pear psylla. The total number of bands were 70, only 61 bands were polymorphic, the percentage of polymorphism between females was (74.49%), females of Al-Rastan and Hama were the most closer (77.22%), the cluster analysis separated the females of\u00a0 Damascus rural in an independent first group, and the second group was separated into two sub clusters, females of Al-Sweida were included in the first sub clusters, and females of other regions were included in the second, as a conclusion, males and females of pear psylla were separated according to geographical distribution and species.<\/p>\n

Key words<\/strong>: Pear psylla, Cacopsylla <\/em>spp., <\/em>Molecular study, ISSR , Syria<\/p>\n

Full paper in Arabic:<\/p>\n

\u00a0\u062f\u0631\u0627\u0633\u0629 \u062c\u0632\u064a\u0626\u064a\u0629 \u0644\u062d\u0634\u0631\u0627\u062a \u0628\u0633\u064a\u0644\u0627 \u0627\u0644\u0623\u062c\u0627\u0635 (Cacopsylla<\/em> spp. (Psyllidae: Hemiptera \u0641\u064a \u0627\u0644\u0645\u0646\u0637\u0642\u062a\u064a\u0646 \u0627\u0644\u0648\u0633\u0637\u0649 \u0648\u0627\u0644\u062c\u0646\u0648\u0628\u064a\u0629 \u0645\u0646 \u0633\u0648\u0631\u064a\u0629<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Bassam Oudeh*(1)\u00a0 Wajih Kassis (2) and Randa Abu-Tara (3) (1). Mokhtaria Research Station, Agricultural Scientific Research Center at Homs, General Commission for Scientific Agricultural Research (GCSAR), Homs, Syria. (2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus, Syria. … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,230],"tags":[154,103,155,46,35],"class_list":["post-477","post","type-post","status-publish","format-standard","hentry","category-plant-protection","category-vol3-no1","tag-cacopsylla-spp","tag-issr","tag-molecular-study","tag-pear-psylla","tag-syria"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/477","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=477"}],"version-history":[{"count":6,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/477\/revisions"}],"predecessor-version":[{"id":701,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/477\/revisions\/701"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=477"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=477"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=477"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}