{"id":4365,"date":"2022-02-16T07:17:23","date_gmt":"2022-02-16T07:17:23","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=4365"},"modified":"2022-02-16T07:17:23","modified_gmt":"2022-02-16T07:17:23","slug":"morphological-and-molecular-characterization-of-rhizoctonia-solani-isolates-causing-black-scurf-disease-to-potato-in-some-regions-of-syria","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=4365","title":{"rendered":"Morphological and Molecular Characterization of Rhizoctonia Solani Isolates Causing Black Scurf Disease to Potato in Some Regions of Syria"},"content":{"rendered":"

Sally Abo Akel(1)<\/sup>, Walid Naffaa(2)*<\/sup>, <\/strong>\u00a0and <\/strong>Mohammed Jamal Mando(1)<\/sup><\/strong><\/span><\/p>\n

(1). Plant Protection Research Admin., General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.<\/span><\/p>\n

(2). Damascus University, Faculty of Agriculture, Department of Plant Protection, Damascus, Syria.<\/span><\/p>\n

(*Corresponding author:Dr. Walid Naffaa. E-mail:\u00a0 walid1851966@yahoo.com<\/a> )<\/span><\/p>\n

Received: 14\/04\/2021\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 10\/05\/2021<\/span><\/p>\n

Abstract<\/strong><\/span><\/p>\n

Potato Solanum tuberosum<\/em> L. is one of the most important food and industrial crops worldwide. Black scurf, caused by Rhizoctonia solani<\/em> K\u00fchn. [teleomorph: Thanatephorus cucumeris <\/em>(A.B. Frank) Donk], is an important disease associated with potato cultivation worldwide including Syria. Nineteen isolates of R. solani<\/em> were obtained from sclerotia formed on infected potato tubers, collected from fields in Homs and Aleppo governorates during the spring season (February, March, & April), and from fields in Damascus countryside (Sa`sa) and Daraa governorates during the summer season (August, & September) in 2018-2019. The isolates varied in their morphological characteristics (colony colour and appearance), ability to form sclerotia, colour of sclerotia and where they were placed on the plate. The molecular study, using a pair of specific primers (ITS4-B \/ AG3-1F) for R. solani<\/em> (AG3) detection, and another pair of specific primers (Rs2R1 \/ Rs1F2) for AG3-PT detection, showed that 78.95% of R. solani<\/em> isolates belonged to the sub-group AG3- PT, except the isolates Rh4, Rh12, Rh14, Rh19 (21.05 %) that might belong to one or more of the non-AG3 anastomosis groups infecting potatoes. Up to our knowledge, this is the first study in Syria for the molecular detection of R. solani<\/em> anastomosis groups. <\/span><\/p>\n

Key words: <\/strong>Rhizoctonia<\/em>, anastomosis groups, black scurf, potato, molecular identification.<\/span><\/p>\n

Full paper in Arabic: pdf<\/a><\/span><\/p>\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Sally Abo Akel(1), Walid Naffaa(2)*, \u00a0and Mohammed Jamal Mando(1) (1). Plant Protection Research Admin., General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria. (2). Damascus University, Faculty of Agriculture, Department of Plant Protection, Damascus, Syria. (*Corresponding author:Dr. Walid Naffaa. E-mail:\u00a0 … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4365","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/4365","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4365"}],"version-history":[{"count":1,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/4365\/revisions"}],"predecessor-version":[{"id":4368,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/4365\/revisions\/4368"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4365"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4365"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4365"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}