{"id":215,"date":"2015-05-26T13:30:04","date_gmt":"2015-05-26T13:30:04","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=215"},"modified":"2016-05-12T05:43:47","modified_gmt":"2016-05-12T05:43:47","slug":"vol2no1p13en","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=215","title":{"rendered":"Evaluation of Some Atriplex Accessions under Salt Stress Conditions and their Molecular Characterization Using ISSR Technique"},"content":{"rendered":"

Basem Alsamman*(1)<\/sup> Yousef Wjhani(1)<\/sup> Marwan Cheikh-Al basatneh(2)<\/sup> and Wael Yousef(1)<\/sup><\/strong><\/p>\n

(1). Biotechnology Department, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria.
\n(2). Damascus University, Faculty of Sciences, Ecology Department.<\/p>\n

(*Corresponding author: Dr. Basem Alsamman, Biotechnology Department, (GCSAR), Damascus, Syria. E-mail:\u00a0 basemhsam1@yahoo.com<\/a>).<\/p>\n

Received: 08\/08\/ 2014\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 09\/03\/ 2015<\/p>\n

Abstract<\/strong><\/p>\n

The study was carried out at Saalow Research Station, in Der Al Zur, General Commission for Scientific Agricultural Research (GCSAR), Der Al Zur, Syria, during the agricultural seasons 2008\/2009- 2009\/2010, 2010\/2011 to evaluate the performance of four accessions related to the genus Atriplex<\/em>, i.e. A. leucoclada, A. canescens, A. halimus<\/em>1 (from Syria) and A. halimus<\/em>2 (from Tunisia) under soil salinity stress conditions depending on a number of morphological indicators. ISSR technique (Inter Simple Sequence Repeats) was used for molecular characterization using 10 primers. The results showed that the studied accessions varied in their response for salinity stress conditions, the average of plant height in A<\/em>triplex <\/em>halimus<\/em>2 (203.3 cm) was significantly higher than the other accessions, A. halimus<\/em>1, A. leucoclada<\/em> and A. canescens<\/em> (177.8, 72 and 64.3 cm), respectively. A. halimus<\/em>1 and A. halimus<\/em>2 revealed the highest tolerance to soil salinity (EC20<\/sub> dSm-1<\/sup>) followed by A. leucoclada<\/em> and lastly A. canescens<\/em>. Results showed vast genetic variation among the studied accessions, all primers proved their effectiveness in showing polymorphism between the studied accessions; primers gave 132 bands with a polymorphic percentage 98%. The number of bands of each primer varied from minimum 7 bands for the primer (ISSR-17899b) to maximum 22 bands in the primer (ISSR-862) with average of 13.2 bands of each primer.<\/p>\n

Key words: <\/strong>Atriplex<\/em>, Soil salinity, Molecular characterization, ISSR.<\/p>\n

Full paper in Arabic :\u00a0\u062a\u0642\u064a\u064a\u0645 \u0628\u0639\u0636 \u0645\u062f\u062e\u0644\u0627\u062a \u0627\u0644\u0631\u063a\u0644 \u062a\u062d\u062a \u0638\u0631\u0648\u0641 \u0627\u0644\u0625\u062c\u0647\u0627\u062f \u0627\u0644\u0645\u0644\u062d\u064a \u0648\u062a\u0648\u0635\u064a\u0641\u0647\u0627 \u062c\u0632\u064a\u0626\u064a\u0627\u064b \u0628\u0627\u0633\u062a\u062e\u062f\u0627\u0645 \u062a\u0642\u0646\u064a\u0629 ISSR<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Basem Alsamman*(1) Yousef Wjhani(1) Marwan Cheikh-Al basatneh(2) and Wael Yousef(1) (1). Biotechnology Department, General Commission for Scientific Agricultural Research (GCSAR), Damascus, Syria. (2). Damascus University, Faculty of Sciences, Ecology Department. (*Corresponding author: Dr. Basem Alsamman, Biotechnology Department, (GCSAR), Damascus, Syria. … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[228],"tags":[100,103,102,101],"class_list":["post-215","post","type-post","status-publish","format-standard","hentry","category-vol2-no1","tag-atriplex","tag-issr","tag-molecular-characterization","tag-soil-salinity"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=215"}],"version-history":[{"count":5,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/215\/revisions"}],"predecessor-version":[{"id":332,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/215\/revisions\/332"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=215"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=215"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}