{"id":1918,"date":"2019-07-02T02:56:06","date_gmt":"2019-07-02T02:56:06","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=1918"},"modified":"2019-07-02T02:56:06","modified_gmt":"2019-07-02T02:56:06","slug":"in-vitro-conservation-of-guava-psidium-guajava-l-var-al-baladi","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=1918","title":{"rendered":"<strong><em>In vitro<\/em><\/strong><strong> Conservation of Guava (<em>Psidium guajava<\/em> L.) var. Al-Baladi<\/strong>"},"content":{"rendered":"<p style=\"text-align: center;\"><strong>Rehab Al Mousa<sup>*<u>(<\/u>1)<\/sup> Neveen Hassan<sup>(2)<\/sup> Ramzy Stino<sup>(3)<\/sup> and Amina Gomaa<sup>(3)<\/sup><\/strong><\/p>\n<p>(1). Department of Biotechnology, <a href=\"http:\/\/www.gewamed.net\/index.php?mod=partners&amp;idp=15\">General Commission for Scientific Agricultural Research<\/a>, Damascus, Syria.<\/p>\n<p>(2). National Gene Bank and Genetic Resources, Agricultural Research Center, Giza, Egypt.<\/p>\n<p>(3). Pomology Department, Faculty of agriculture, Cairo University, Giza, Egypt.<\/p>\n<p>(*Corresponding author: Dr. Rehab Al Mousa. E-Mail: <a href=\"mailto:bebo_moussa13@yahoo.com\">bebo_moussa13@yahoo.com<\/a>).<\/p>\n<p style=\"text-align: center;\">Received: 04\/01\/2018\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Accepted: 06\/02\/2018<\/p>\n<p style=\"text-align: center;\"><strong>Abstract<\/strong><\/p>\n<p style=\"text-align: justify;\">This study was conducted at the National Gene Bank, Egypt, from 2012 to 2014; to find out <em>in vitro<\/em> conservation protocol of guava (<em>Psidium guajava <\/em>L. var. Al-Baladi). The medium-term conservation study was initiated at 10 and 15\u00b0C under complete darkness conditions. Shoot tip explants were cultured on MS medium with different concentrations (3.0, 4.0, 5.0 and 6.0%) of sucrose or sorbitol. After 6 months of conservation, up to 83.33% of explants survived on medium with 5.0% sorbitol at 15\u00baC, while the same survival% (66.67%) was noticed when explants conserved on medium with 6.0% of sorbitol at 15\u00baC and on medium with 5.0 or 6.0% of sorbitol at 10\u00baC. Regeneration%, shoot number of explant and shoot length were not affected by osmotic concentrations. Meanwhile, all explants lost their ability to regenerate new shoots when conserved for 6 months on medium with 3.0% sucrose (control) at 10\u00baC. Guava seeds recorded 100% germination when desiccated for 6 h before cryopreservation in liquid nitrogen.<\/p>\n<p><strong>Keywords:<\/strong> Guava, Medium-term conservation, Sucrose, Sorbitol, Cryopreservation, Desiccation.<\/p>\n<p>Full Paper in Arabic: <a href=\"http:\/\/agri-research-journal.net\/sjar\/wp-content\/uploads\/2019\/07\/v6n2p2.pdf\">PDF<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Rehab Al Mousa*(1) Neveen Hassan(2) Ramzy Stino(3) and Amina Gomaa(3) (1). Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria. (2). National Gene Bank and Genetic Resources, Agricultural Research Center, Giza, Egypt. (3). Pomology Department, Faculty of agriculture, &hellip; <a href=\"https:\/\/agri-research-journal.net\/SjarEn\/?p=1918\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[636,651],"tags":[497,764,762,763,631,585],"class_list":["post-1918","post","type-post","status-publish","format-standard","hentry","category-hurticulture-2","category-v6n2-2019","tag-cryopreservation","tag-desiccation","tag-guava","tag-medium-term-conservation","tag-sorbitol","tag-sucrose"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1918","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1918"}],"version-history":[{"count":1,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1918\/revisions"}],"predecessor-version":[{"id":1919,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1918\/revisions\/1919"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1918"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1918"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1918"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}