{"id":1211,"date":"2017-09-30T08:51:07","date_gmt":"2017-09-30T08:51:07","guid":{"rendered":"http:\/\/agri-research-journal.net\/SjarEn\/?p=1211"},"modified":"2017-11-16T11:33:35","modified_gmt":"2017-11-16T11:33:35","slug":"a-study-of-inhibition-activity-of-lactobacillus-spp-against-fungi-and-aflatoxin-b1-in-vitro","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=1211","title":{"rendered":"<strong>A Study of Inhibition Activity of <em>Lactobacillus<\/em> spp. Against Fungi and Aflatoxin B1 in Vitro<\/strong>"},"content":{"rendered":"<p style=\"text-align: center;\"><strong>Keethar Rashid Majeed<sup>(1)<\/sup>, Amal Khazem Ghadban<sup>(1)<\/sup> and Faleh Mehdi Saleh<sup>*(1)<\/sup><\/strong><\/p>\n<p style=\"text-align: center;\">(1). Food technology Department, Factual of Agriculture, Al Basra University, Al Basra, Iraq.<\/p>\n<p style=\"text-align: center;\">(*Corresponding author: Dr. Faleh Mehdi Saleh. E-Mail: husienforever1@gmail.com).<\/p>\n<p style=\"text-align: center;\">Received: 27\/07\/2017\u00a0\u00a0 \u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Accepted: 18\/08\/2017<\/p>\n<p style=\"text-align: center;\"><strong>Abstract <\/strong><\/p>\n<p style=\"text-align: justify;\">This study was conducted in April 2015 to August 2016, to investigate the inhibition activity of <em>Lactobacillus<\/em> spp. in vitro against fungi and aflatoxin using local isolates i.e., <em>L. casi<\/em> (AKF <sub>1<\/sub>), <em>L. plantarum<\/em> (AKF<sub>2<\/sub>), <em>L. casi<\/em> (AKF <sub>3<\/sub>), <em>L. plantarum<\/em> (AKF <sub>4<\/sub>) and <em>L. Jonsonii<\/em> (AKF <sub>5<\/sub>). The results of the statistical analyses showed that AKF<sub>4<\/sub> exhibited the highest inhibition activity (97, 62.33 and 97%) among all isolates using live bacteria, thermally killed bacteria, and its metabolic compounds, respectively. In contrasts, the inhibition activity of AKF<sub>3<\/sub> were (59, 14.33 and 59%) using live bacteria, thermally killed bacteria, and its metabolic compounds, respectively. Furthermore, aflatoxin binding was studied in vitro using the isolates (AKF <sub>1,2,3,4,5<\/sub>) in MRS media containing 200 ppb of aflatoxin B<sub>1<\/sub>. The results revealed that using live bacteria of AKF<sub>4<\/sub> at concentration 1\u00d710<sup>8<\/sup> cfu\/ml for 48 h showed highest binding percentage 99.9 %, while thermally killed bacteria of AKF<sub>1,2,5<\/sub> at concentration 500 ppb for 48 h gave the highest binding percentage 100 % at 37 \u00b0C and pH 6.5. In addition, AKF 4 had the highest binding percentage 100% for both 24 h and 48 h at the same conditions. Binding efficiency results of thermally killed bacteria was 100 %, while binding efficiency of live bacteria ranged between (99.87-100%) when the bacteria was washed with water, acetonitrile and phosphate buffer saline.<\/p>\n<p><strong>Keywords<\/strong>: Wheat, <em>Lactobacillus<\/em>, Aflatoxin Binding B1, Fungal growth inhibition.<\/p>\n<p><strong>Full paper in Arabic:<a href=\"http:\/\/agri-research-journal.net\/sjar\/wp-content\/uploads\/2017\/11\/v4n3p6.pdf\"><span style=\"color: #ff0000;\"> PDF<\/span><\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Keethar Rashid Majeed(1), Amal Khazem Ghadban(1) and Faleh Mehdi Saleh*(1) (1). Food technology Department, Factual of Agriculture, Al Basra University, Al Basra, Iraq. (*Corresponding author: Dr. Faleh Mehdi Saleh. E-Mail: husienforever1@gmail.com). Received: 27\/07\/2017\u00a0\u00a0 \u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0Accepted: 18\/08\/2017 Abstract This study &hellip; <a href=\"https:\/\/agri-research-journal.net\/SjarEn\/?p=1211\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,407],"tags":[428,429,427,11],"class_list":["post-1211","post","type-post","status-publish","format-standard","hentry","category-plant-protection","category-vol4-no3-2017","tag-aflatoxin-binding-b1","tag-fungal-growth-inhibition","tag-lactobacillus","tag-wheat"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1211","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1211"}],"version-history":[{"count":5,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1211\/revisions"}],"predecessor-version":[{"id":1275,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/1211\/revisions\/1275"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1211"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1211"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}