{"id":10266,"date":"2025-10-24T08:10:19","date_gmt":"2025-10-24T08:10:19","guid":{"rendered":"https:\/\/agri-research-journal.net\/SjarEn\/?p=10266"},"modified":"2025-10-24T08:11:06","modified_gmt":"2025-10-24T08:11:06","slug":"assessment-of-the-tolerance-of-some-grapevine-rootstocks-to-drought-stress-treatments-using-plant-tissue-culture-technology","status":"publish","type":"post","link":"https:\/\/agri-research-journal.net\/SjarEn\/?p=10266","title":{"rendered":"Assessment of the tolerance of some grapevine rootstocks to drought stress treatments using plant tissue culture technology"},"content":{"rendered":"

Mohsen*(1)<\/sup>, Kh. AL-kountar(1)<\/sup>, M.Amer(1)<\/sup><\/strong> and O. Aljbaii(2)<\/sup><\/strong><\/span><\/p>\n

(1). General Commission for Scientific Agricultural research, Al- Suwayda Scientific Agricultural Research Center<\/span><\/p>\n

\u00a0(2). Damascus University<\/span><\/p>\n

\u00a0(*Corresponding author: Wasim Mohsen, email: wasimmo6@yahoo.com<\/a> )<\/span><\/p>\n

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Received:\u00a0\u00a0 22\/11\/2024\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Accepted:\u00a0 6\/4\/2025<\/span><\/p>\n

Abstract:\u00a0<\/strong><\/span><\/p>\n

The research was carried out in Al-Suwayda research center during the period 2016-2019, seven American rootstocks of grapevine which is resistant to Phylloxera insect have been studied in vitro<\/em> for its response to drought stress treatments, these rootstocks are775P VGVA 12, 140Ruggeri UBA05, 161 49 VGVA5, 157 11C UBA01 1103 Paulsen UBA01, Kober 5BB\u00a0 UBA01, 779P UBA04. polyethylene glycol ( PEG6000 <\/sub>) has been used for drought stress treatments, explants between 2-3 cm with two leaves have been planted in WPM liquid medium with ( 1mg\/L Kin + 0.2 mg\/L IBA + 0.2 mg\/L GA3( \u00a0and added to it ( PEG6000 <\/sub>) in this concentrations 0,2,4,6, and 8%, with paper bridges used to carry explants, the results were taken 60 days after planting for the rate of survival plants, shoot length, shoots number, leaves number, roots length, roots number and the extent of drought damage,\u00a0\u00a0 The results show a difference in rootstocks response to water stress based on the studied parameters. Progressive reduction in the studied parameters occurred as PEG6000<\/sub> concentration increased compared with control., where each of the rootstocks, 140 Ruggeri, 775\/p, 157\/11C and Kober 5BB is the lowest decrease in most of the studied indicators. This indicates their good tolerance of the applied drought stress, while the rootstocks, 779\/P 161\/49 could not tolerant the drought stress well compared to the rest of the studied rootstocks, as the studied indicators decreased significantly.\u00a0 The highest percentage of plants in plants was 8% PEG6000. Kober 5BB rootstock record a high level in shoots length reaching 6.78 cm on 2% PEG6000<\/sub>, treatment, it also recorded the average number of leaves was 7.2 leaf on the same one, while it did not register any significant difference between the control 2% PEG6000 <\/sub>treatment for shoots number, but for roots length it has recorded an average 9.7 cm. while 140Ruggeri did not register any significant difference between the control and the treatments for roots number excluding the treatment containing 8% PEG6000 <\/sub>which has recorded an average 1.01 root. drought injury varied depending on the rootstock and PEG6000<\/sub> concentration, the deadest plants were observed on 8% PEG6000<\/sub>, the results show that Kober 5BB rootstock has the lowest level of injury degree reaching 1.3, while 161 49 rootstock has the highest level of injury degree reaching 1.8.<\/span><\/p>\n

Keywords:<\/strong> American grape origins, dry stress, plant tissue transplantation.<\/span><\/p>\n

\u00a0Full paper in Arabic: PDF<\/a><\/span><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Mohsen*(1), Kh. AL-kountar(1), M.Amer(1) and O. Aljbaii(2) (1). General Commission for Scientific Agricultural research, Al- Suwayda Scientific Agricultural Research Center \u00a0(2). Damascus University \u00a0(*Corresponding author: Wasim Mohsen, email: wasimmo6@yahoo.com ) \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Received:\u00a0\u00a0 22\/11\/2024\u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-10266","post","type-post","status-publish","format-standard","hentry","category-sjar"],"_links":{"self":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/10266","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10266"}],"version-history":[{"count":3,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/10266\/revisions"}],"predecessor-version":[{"id":10271,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=\/wp\/v2\/posts\/10266\/revisions\/10271"}],"wp:attachment":[{"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10266"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10266"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agri-research-journal.net\/SjarEn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10266"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}