Ghufran Hujaira* (1), Omiema Nasser (1), and Nabila Kreidi (2)
(1). Department of Environmental Protection, Higher Institute for Environmental Research, Tishreen University, Lattakia, Syria.
(2). General Commission for Scientific Agricultural Research, Damascus, Syria.
(*Corresponding author:Ghufran Hujaira, E-mail: dr.mbenmahmoud@yahoo.com).
Received:14/02/2022 Accepted:26/05/2022
Abstract:
In order to obtain pure isolates of atmospheric nitrogen-fixing Azospirillum bacteria from local soil, and testing their efficiency in the fixation process for use as a bio-fertilizer, ten bacterial strains were isolated from soil belonging to several sites in Hamima area in Aleppo governorate. Experiments were conducted at the Agricultural Research Center in Aleppo on the date of 2020, the effectiveness of these isolates was tested in fixing atmospheric nitrogen by inoculating them on nitrogen-free liquid environments, with a witness that was not inoculated with bacteria. The results showed the superiority of three isolates with atmospheric nitrogen fixation, where the percentage of atmospheric nitrogen fixation for the sixth, fourth and third three isolates, respectively, reached (12.4) (8.7) (6.2) mg N/g, while the characterization results showed that the sixth bacteria is Azospirillum brasilense, and the fourth is Azospirillum lipoferum, the third is Azospirillum amazonense.
Key words: nitrogen-fixing bacteria, Azospirillum, intimate soil, nitrogen.
Full paper in Arabic: pdf