Salih Hassan Al-Azzawi ⁽¹⁾and Abdulmuttaleb Hazim khaleel⁽¹⁾

(1). Department of Animal Production،College of agriculture، University Diyala،،Iraq.

(*Corresponding author: Abdulmuttaleb khaleel E-mail: abdmtaleb85@gmail.com)

Received:15/12/2021                 Accepted:9/05/2022

Abstract: 

The heat shock protein 70 (HSP70) gene is important as it affects the heat endurance of animals through its participation in the protein folding process. The current study was conducted at the ruminant farm of the College of Agriculture at the University of Diyala. This study aimed to demonstrate the effect of a single polymorphism in the sequence encoding the heat shock gene HSP70 on some hematological and biochemical characteristics of Iraqi male goats. The DNA sample isolated from 15 male goats was amplified by PCR technique for the coding region on the HSP70 gene. Single nucleotide polymorphism (SNP) sequencing was used to detect the DNA sequence in which a site mutation (1528 C/T) was detected. Hematological characteristics (PCV bound cell volume, hemoglobin Hb concentration, RBC count, WBC count, MCV, mean hemoglobin in MCH and the mean amount of hemoglobin in the MCHC group) were also evaluated. and biochemical characteristics alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate amino transferase (AST). The results showed the presence of three genotypes CC, CT and TT with percentages (0.07, 0.40, 0.53), respectively, and the frequency of alleles C and T (0.27, 0.73 respectively) and in Hardy Weinberg equilibrium. The animals were under severe heat stress and an increase in the number of respirations was noted And the afternoon rectal and skin temperature in animals with the genotype CC for the month of July and the number of respirations for the month of August in the afternoon, as it was noted a decrease in the number of red blood cells for the CT group in the afternoon for the month of July and a decrease in the aminotransferase enzyme (AST) for the CC group. The TT genotype was significantly superior to the red blood cell count in the afternoon, and a significant decrease in the aminotransferase enzyme (AST) of the CC group was observed in the morning measurement, but the afternoon measurement was the lowest TT group in this trait. As for the differences within the groups, most of them were significant for the studied traits.

Key words: HSP70, hematological, biochemical, goats

Full paper in Arabic:  pdf