Enhancing an In Vitro Shoot Formation of Ixora coccinea L. Using Silver Nitrate

Rania Ahmed Hassan Onsa(1)*, Igbal Abd Elgadeir Abd Ellatif (2), Magdoleen Gamar Eldeen Osman(3)

(1). Department of Horticulture, Faculty of Agriculture, University of Al zaiem Al azhari , Khartoum, Sudan

(2). Department of Horticulture, Faculty of Agriculture Studies, Sudan University of Science and Technology, Khartoum, Sudan

(3). Plant Tissue Culture, National Center of Research, Khartoum, Sudan.

(*Corresponding author: Dr. Rania Ahmed Hassan Onsa, E-mail: raniaonsa@gmail.com)

       Received: 16/12/ 2021            Accepted: 16/03/ 2022


Ixora coccinea is a popular ornamental plant, as well as it is an important medicinal plant. This study was conducted to study the effect of silver nitrate (AgNO3) on shoot formation of Ixora coccinea. Shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.0, 2.5, 5.0, 8.0 and 10.0 mg/l) of AgNO3 for establishment stage. Then shoot tip explants sub-cultured on MS medium supplemented with 8.0 mg /l AgNO3 combined with different concentrations (0.0,0.5,1.0 and 2.0 mg/l) of Benzyl aminopurine (BAP) for multiplication stage. The results showed that uses of different concentrations of AgNO3 alone affect positively on shoot morphogenesis of I. coccinea. 8.0 mg /l of AgNO3 gave the best result on shoot formation. However, 5.0, 8.0 and 10.0 mg/l AgNO3 scored similar results in number of nodes. The combine of 8.0 mg /l AgNO3 and 2.0 mg/l BAP to MS medium enhanced the multiplication rate of shoot significantly.

Keywords: AgNO3, In vitro, Ixora coccinea, Micropropagation, Shoot formation.

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