Rania Ahmed Hassan Onsa^(1)*, Igbal Abd Elgadeir Abd Ellatif ⁽²⁾, Magdoleen Gamar Eldeen Osman⁽³⁾

(1). Department of Horticulture, Faculty of Agriculture, University of Al zaiem Al azhari , Khartoum, Sudan

(2). Department of Horticulture, Faculty of Agriculture Studies, Sudan University of Science and Technology, Khartoum, Sudan

(3). Plant Tissue Culture, National Center of Research, Khartoum, Sudan.

^(*Corresponding author: Dr. Rania Ahmed Hassan Onsa, E-mail: raniaonsa@gmail.com)

       Received: 16/12/ 2021            Accepted: 16/03/ 2022

Abstract: 

Ixora coccinea is a popular ornamental plant, as well as it is an important medicinal plant. This study was conducted to study the effect of silver nitrate (AgNO₃) on shoot formation of Ixora coccinea. Shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.0, 2.5, 5.0, 8.0 and 10.0 mg/l) of AgNO₃ for establishment stage. Then shoot tip explants sub-cultured on MS medium supplemented with 8.0 mg /l AgNO₃ combined with different concentrations (0.0,0.5,1.0 and 2.0 mg/l) of Benzyl aminopurine (BAP) for multiplication stage. The results showed that uses of different concentrations of AgNO₃ alone affect positively on shoot morphogenesis of I. coccinea. 8.0 mg /l of AgNO₃ gave the best result on shoot formation. However, 5.0, 8.0 and 10.0 mg/l AgNO₃ scored similar results in number of nodes. The combine of 8.0 mg /l AgNO₃ and 2.0 mg/l BAP to MS medium enhanced the multiplication rate of shoot significantly.

Keywords: AgNO₃, In vitro, Ixora coccinea, Micropropagation, Shoot formation.

Full paper in English: pdf