Laboratory Study of some Characteristics of some Fungal Isolates of Alternaria alternata, Alternaria solani and Botrytis cinerea and enzymatic properties and Sensitivity to Culture Filtrate

Nsreen Dib(1)*  Hany Ashour Badawy(2) Ahmed Abdel Kader Ashour(2)  Tomader gomaa Abel rahman(3)

(1). Agricultural Scientific Research Center, Latakia.

(2). faculty of Agriculture , Cairo University, Egypt.

(3). plant pathology institute, Agricultural Research Center, Egypt.

(*Corresponding author: Nsreen Did  . E-Mail:

Received: 4/01/2022          Accepted:13/03/2022


This experiment was carried out at the laboratory of the plant diseases institute, agriculture scientific research center, Giza, Egypt in the season 2016-2017. And this study aimed to study the mycelium growth characteristics  leaf spot disease caused by A. alternata , early blight disease caused by A. solani and gray mold disease caused by B. cinereal on different culture medium. The bioassaying to the filtrate of the culture media which the tested isolates grow and identification of mycotoxins in the filtrate of the culture media. The results revealed that the radial growth test results significantly differed between the high and less aggressive isolates. The high aggressive isolates were grew faster than the less aggressive isolates on all tested media. The enzyme activity (PG and CX) of the high aggressive isolates were higher than that in the less aggressive isolates of tested fungi. Five toxins; Altenuene (ALT), Altertoxin (ATX), Alternariol (AOH), Alternariol methyl ether (AME) and Tenuazonic acid (TeA) were detected in the culture filtrate extracts of A. alternata and A. solani,  in addition to Alternaric acid, which was produced by A. solani. A total of 14 metabolites were detected in the culture filtrate of the high and less aggressive isolates of B. cinerea. Botcinolide was found only in the cultures of the aggressive isolates of B. cinerea; BcB-2, BcB-1 and BcI-2. The culture filtrate extracts of the high aggressive isolates of A. alternata, A. solani and B. cinerea had more phyto- and mycotoxins than the less aggressive isolates.

Key words: Alternaria alternata, Alternaria solani, Botrytis cinerea, culture filtrate, fungal toxins.

Full paper in Arabic: pdf