Effect of some Methods for Storing Sour Orange and Cleopatra Mandarine Pollen and its Viability and Ability to Germinate

Venus Hasan*(1) , Ali Elkhateeb(1) and Hasan Khojah(2)

(1). General Commission Agriculture Scientific Research, Lattakia Syria.

(2). Horticulture Department, Agriculture College, Tishreen University, Syria.

(*Corresponding author: Venus Hasan. E-Mail: venushasan80@gmail.com).

Received: 28/12 /2021                Accepted: 23/02/2022


The study was conducted during the 2019-2020 season at the Scientific Agricultural Research Center in Lattakia, the aim is to determine the best storage method for pollen grains of two citrus rootstocks, sour orange, and Cleopatra mandarine, and test their vitality and ability to germinate for use in hybridization programs throughout the flowering season. The flower buds were picked in the balloon stage and divided into two parts. flower buds and anthers, three storage methods were tested, 4°C, -20°C, and for three periods of 7, 15 and 30 days, then viability and germination tests were conducted in each storage stage, Germination was studied laboratory temperatures (20 ± 2) C, and incubator temperature (24 ± 2) C. and on three culture media, distilled water, sucrose solution 15%, sucrose solution 30%, The result was that pollen gives the highest percentage of germination immediately after picking and the best way to store pollen is to store pollen at low temperature, the method of storing the anthers was significantly superior to storing the buds at the cold temperature, the temperature of-20°C was superior to 4 °C, and the dye gave an idea of the vitality of the pollen, but did not express its ability to germinate.

Keywords: pollen, storage, vitality, sour orange, Cleopatra mandarin.

Full paper in Arabic: pdf