Reem Hamid(1), Faisal Bakkour(1) and Fadi Abbas*(2)
(1). Department of Field Crops, Faculty of Agriculture, Al Baath University, Homs, Syria.
(2). General Commission for Scientific Agricultural Research (GCSAR), Agriculture Research Center of Homs, Homs, Syria.
(*Corresponding author: Dr.Fadi Abbas. E-Mail: fadiab77@gmail.com).
Received: 22/05/2021 Accepted: 16/08/2021
Abstract
This experiment was conducted in the labs of General Commission for Scientific Agricultural Researches GCSAR, Homs Center, during 2020. PEG6000 was applied to the germination media with low osmotic potentials (OP) i.e. 0, -0.2, -0.4, -0.6, -0.8, MPa, for screening 15 chickpea genotypes under controlled conditions (in incubator at 20±3 C, constant humidity 70% RH) . Complete randomized design (CRD) with three replicates was used. The germination %, germination speed, radicle and hypocotyl length, and seed vigor were studied, and the genotypes classified according to these attributes, relative drought stress indices, and Z. distribution. The results exhibited a genetic difference between the genotypes concerning the response to the application of PEG6000 in the media. The germination percent was decreased with the decline of osmotic potential 0, -0.2, -0.4, -0.6, -0.8, MPa, the percent of germination were (100, 87.11, 73.78, 54.11, 20.67 %) respectively, as compared with the control. While the speed of germination was (2.92, 2.14, 1.59, 0.83, 0.38) respectively. The length of epicotyl and radicle were estimated in all of tested osmotic potentials, the results showed that with decreasing the osmotic potential the length decreased, and the decrement in epicotyl was more than radicle. And seed vigor decreased with OP decreasing. According to the differences between genotypes in the response to drought stress using PEG6000 during germination, RDTI indices, and the z-distribution analysis, the genotypes were classified to tolerant to drought stress, i.e., Gab4, FLIP87-59C FLIP87-8C, and Gab5. and could be sown successfully under the most drought conditions, compare to other genotypes. While the genotypes Turkish, Morocco, FLIP12-250C, ILC3279, and FLIP08-42C classified the most sensitive,
Keywords: Osmotic Potential, Polyethylene Glycol PEG6000, Germination Stage, Chickpea.
Full paper in Arabic: pdf