Biodegradation of Phenol in Olive Mill Waste Water Using Local Isolated Microorganism

Nabila kridi* (1) Muhammad Manhal AL-Zoubi (1) Muhammud Abogora(2)Mohamed Said Al-Shater (2)

(1). Administration of Natural Resources Research, General Commission for Scientific Agricultural Research, (GCSAR), Damascus, Syria

(2). Department of Plant Protection, Faculty of Agriculture, Damascus University, Damascus,  Syria   

(*Corresponding author: Nabila kride. E-Mail: nabilakridi@hotmail.com).

Received: 24/3/2020          Accepted: 08/ 07/2020

Abstract

 The availability of large quantities of olive mill waste water and the difficulty of disposal, and the random addition of agricultural soils as organic fertilizer led to the contamination of the soil with phenolic compounds and poisoned plants and microorganisms. Phenolic compounds olive mill waste water isolated from olive mill waste water and saturated soils, and from colonies that appear on the roofs of pots keep the olives home. The ability of isolated microorganisms to dismantle phenolic compounds was tested by planting them in nutrient media containing phenolic compounds as the sole carbon source. The ten isolates were incubated with unsterile Olive Mill Waste Water and after sterilization by filtration method for 21 days with a control sample without isolation.

 The results showed that isolated microorganisms were able to more the proportion of phenolic compounds decompose to the control in different proportions, the (non-sterile) was able to decompose the phenolic compounds by 7.78% compared to the sterilizer. The fungus also outperformed the bacteria in its ability to reduce the concentration of phenols, where Penicillium sp significantly outperformed the other isolates in olive mill waste water (sterile and non-sterile), followed by Aspergillus flavos, then Pseudomonas sp and Bacillus sp. The rest of the isolates were less effective in the decomposition. The most effective isolates can be used to reduce the content of olive mill waste water from toxic phenolic compounds and the biological treatment of soils contaminated with this substance.

 Keywords: Biodegradation, bacteria, fungi, olive mill waste water, phenol

Full paper in Arabic: PDF