Rawaa Aoush* ⁽¹⁾, Faysal Bakkour ⁽²⁾ and Lina AL-Nadaf ⁽²⁾

(1). Biotechnology Research Center, Homas, Syria.

(2). Faculty of Agriculture , Albaath University Homas, Syria.

(*Corrsponding author: Rawaa Aoush. E-Mail: rawaayoush1987@gmail.com).

Received: 16/09/2020                                Accepted: 28/01/2021

Abstract

Chickpea (Cicer arietinum.L) is one of the most important leguminous crops in the world, drought is one of the most important abiotic stress that limit plant production as a whole, and it causes morphological, physiological, biochemical and molecular changes in plants, field study was carried out in the village of Jandar in  Homs, and chemical analyzes were conducted at the Biotechnology Research Center of Al-Baath University in 2019, with the aim of studying the change in some biochemical characteristics (the content of the leaves of proline (pro) – hydrogen peroxide (H₂O₂) – the activity of the enzyme catalase (CAT). Under drought stress, and to determine the possibility of adopting these indicators as a rapid detection method for the tolerant genotypes for later use in breeding programs. Seeds were sown in polyethylene pot and placed under a transparent canopy to avoid rain, completely randomized design was used in the experiments (CRD), and with three replications, and Irrigation was carried out regularly when ground moisture reached to 75% of the field capacity of non-stressed plants and 40% for stressed plants. The aforementioned indicators were studied at the flowering stage, and the results showed a significant increase in all the studied traits under stress when compared to the irrigated control. The tolerant Genotypes (Gap 5 and Gap 4) showed a significantly higher percentage of catalase increase compared to the two sensitive models and a lower percentage increase of free radical hydrogen peroxide (H2O2) under stress conditions, Whereas the role of proline varied between genotypes, it was significantly lower in the tolerant genotype GAP5  and significantly higher in the sensitive genotype LIC3279, while its content was significantly higher for the tolerant genotype GAP 4 compared to the sensitive Genotype Spring Municipal which indicates that the role that proline plays when exposed to stress It should be supported by an integrated antioxidant system as it is either evidence of tolerance or anaphylaxis and depletion of photosynthetic products.

Keyword: Chickpeas – Drought stress – Catalase – Proline – Hydrogen peroxide.

Full paper in Arabic: PDF