Louna Rizk Allah Kasoha ^*1

(1) Biotechnoloy center, Faculty of Agriculture, University of Aleppo, Syria.

 (*Corrsponding author: Louna Kasoha .E-Mail: Lonaty99@hotmail.com).

Received: 31/08/2020                               Accepted: 22/11/2020

Abstract

This research was conducted to produce oyster mushroom spawn using 12 different grain kinds including wheat grain as control, barley, chickpea, lentil, pea, and mixture of wheat +  barley, wheat + chickpea, wheat +  lentil, wheat +  pea, barley +  chickpea, barley +  lentil, and  barley +  pea in rating 50%, to study the effect of these grain spawn on the mycelium growing all over the substrate, moreover, to study the effect of spawn produced from those grain on the productivity on wheat straw substrate and its biological efficiency. The experiments were conducted at Aleppo University – Biotechnology centre, during 2019-2020. The strain 3015 was cultivated on 12 grain mixture types. The experiment was conducted in a complete randomized blocks design, and data were statistically analysed using GENSTAT program and ANOVA tables were calculated. LSD0.05 were used to compare among the means based on each experiment.

The results showed that grain kinds had clear effect on spawn on grain substrate, wheat substrate, and productivity as spawn completed growth after 5.8 days on 50 % barley + 50 % pea and pea substrates which was the shortest and significantly differences from the other grain kinds. Also they were the fastest on spawn growth on wheat substrate as they reach 9.97 and 10.27 days, respectively, and they achieved the highest production of 404.61 and 383 g/kg, respectively. They also gave biological efficiency of 101.15 % and 95.76 %, respectively. Results also showed that spawn growth on substrate with two grain types took shorter time in comparison with growing in substrate consist of one type of grain, which made the two grain kinds substrate enter production stage early and gave higher oyster mushroom production. All these grain substrates were significantly superior wheat grain substrate in production quantity and biological efficiency (higher than 80%), so all these grain spawns were suitable to produce oyster mushroom spawn instead of using wheat grains alone.

Keyword: Grain spawn, Mixtures, oyster mushroom spawn, Mycelium, and productivity.

Full paper in Arabic: PDF