The Effect of Bacterial Inoculation (Rhizobium ‎leguminosarum) on Growth and Production Traits of ‎Lentil

Alaa Abdel Halim Habib*(1) and Issa Noor Aldeen Kabibou(1)

(1). Department of Soil and Water Sciences, Faculty of Agriculture, Tishreen University, Lattakia, Syria

(*Corresponding author: Eng. Alaa Abdel Halim Habib.

Received: 04/11/2019                               Accepted: 7/12/2019


The research was carried out at Alqanjara village in Latakia governorate, during 2018/2019 in order to compare the effect of adding two locally isolated strains and a imported strain of rhizobium on lentil growth and productivity to select the most efficient strain in nitrogen fixation. The experiment was applied according to randomized complete block design (RCBD) in split plot arrangement with three replicates, nitrogen fertilization factor was assigned to the main plots and bacterial inoculation treatments were allotted to the subplots. The research included (15) treatments, in which the bacterial inoculation treatments were: without inoculation, inoculation of the first strain isolated from rosy nodes of faba bean plant roots, inoculation of the second strain isolated from rosy nodes of lentil roots, inoculation of mix of the first and second strains and inoculation of the third strain which is a commercial strain imported under the name of Okadin bio-fertilizer. Phosphate and potassium fertilizers were added to all treatments at a rate of 25 kg/d triple in the form of superphosphate 46%, 25 kg/d of potassium sulphate 50%. While the nitrogen fertilizer was added according to three levels: without addition and 5 kg/d and 10 kg/d (Urea 46%), to detect its effect on bacterial activity and its ability to fix atmospheric nitrogen. The results showed that the bacterial inoculation with strain3 (Okadin bio-fertilizer) had a positive effect on plant growth and an increase in the number of nodules by 345% compared to the non-inoculated (control). Bacterial inoculation treatments of the third strain with the addition of nitrogen fertilizers at level two had shown a significant effect in improving crop quality. The increase in productivity was estimated at 446% compared to the control, and 92% and 231% compared to the two fertilization treatments alone, while the number of nodules was increased by 302% and 295%, dry shoots weight was increased by 194% and 369% compared to the two fertilization treatments alone. Inoculated treatments of the third strain exceeded those in the second sand the first strains, where the increase in productivity reached 13% compared with the second strain and 67% compared with the first strain. The total nitrogen content in the soil increased compared to the control treatment, and the PH value decreased slightly in inoculated treatments compared to pre-planting, with the increase of the available P concentration from 22 to 29 ppm and a slight decrease in electrical conductivity from 0.75 to 0.46 ds/m.

Key words: Rhizobium, Lentil, Bacterial inoculation, Nitrogen Fertilizer .

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