Rehab Al Mousa*(1) Neveen Hassan(2) Ramzy Stino(3) and Amina Gomaa(3)
(1). Department of Biotechnology, General Commission for Scientific Agricultural Research, Damascus, Syria.
(2). National Gene Bank and Genetic Resources, Agricultural Research Center, Giza, Egypt.
(3). Pomology Department, Faculty of agriculture, Cairo University, Giza, Egypt.
(*Corresponding author: Dr. Rehab Al Mousa. E-Mail: firstname.lastname@example.org).
Received: 04/01/2018 Accepted: 06/02/2018
This study was conducted at the National Gene Bank, Egypt, from 2012 to 2014; to find out in vitro conservation protocol of guava (Psidium guajava L. var. Al-Baladi). The medium-term conservation study was initiated at 10 and 15°C under complete darkness conditions. Shoot tip explants were cultured on MS medium with different concentrations (3.0, 4.0, 5.0 and 6.0%) of sucrose or sorbitol. After 6 months of conservation, up to 83.33% of explants survived on medium with 5.0% sorbitol at 15ºC, while the same survival% (66.67%) was noticed when explants conserved on medium with 6.0% of sorbitol at 15ºC and on medium with 5.0 or 6.0% of sorbitol at 10ºC. Regeneration%, shoot number of explant and shoot length were not affected by osmotic concentrations. Meanwhile, all explants lost their ability to regenerate new shoots when conserved for 6 months on medium with 3.0% sucrose (control) at 10ºC. Guava seeds recorded 100% germination when desiccated for 6 h before cryopreservation in liquid nitrogen.
Keywords: Guava, Medium-term conservation, Sucrose, Sorbitol, Cryopreservation, Desiccation.
Full Paper in Arabic: PDF